DNA 형광 염색을 이용한 치은열구상피부착 세균에 관한 연구
Fluorescent detection of bacteria associated with gingival sulcus epithelium
신승윤, 계승범, 양승민, 이상현,
소속 상세정보
신승윤 ( Shin Seung-Yun ) - 성균관대학교 의과대학 삼성서울병원 치과진료부 치주과
계승범 ( Kye Seung-Beom ) - 성균관대학교 의과대학 삼성서울병원 치과진료부 치주과
양승민 ( Yang Seung-Min ) - 성균관대학교 의과대학 삼성서울병원 치과진료부 치주과
이상현 ( Lee Sang-Hyun ) - 성균관대학교 의과대학 삼성서울병원 치과진료부 치주과
KMID : 0363020080380040639
Abstract
Purpose: The aim of this study was to compare the number of live and dead bacteria attached to, or within, the stratified squamous epithelium lining the tissue side of the gingival sulcus.
Materials and Methods: A total of 50 patients was examined and classified into healthy or diseased sites according to inflammatory status of the gingival tissue. The surface of stratified squamous epithelium was removed by gentle scraping of the gingival sulcus with curettes. The cells were processed in the laboratory by density-gradient centrifugation to separate the epithelial cells from the loose bacteria and debris. The LIVE/ Bacterial Viability Kit was applied and the specimens were observed by an epifluorescent microscope and the number of bacteria was counted.
Results: Live and dead bacteria were stained to green and red, irrespectively. Generally, the number of total bacteria in the diseased sites was significantly higher than in the healthy sites. The mean number of detected bacteria in the diseased sites was (red bacteria / green bacteria ), while it was in the healthy sites (red bacteria / green bacteria ). The percentage of red bacteria was in the diseased sites and in the healthy sites.
Conclusion: The total number of bacteria in the diseased sites was significantly higher than that of the healthy sites. The ratio and the number of red bacteria were also significantly higher in the diseased sites.
키워드
bacterial adhesion;gingival sulcular epithelium;bacterial viability
원문 및 링크아웃 정보
등재저널 정보